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Khối Van Phân Phối Chất Lỏng Chính Xác HPLC Bằng PEEK & Thép Không Gỉ 316L Tùy Chỉnh
Custom virgin PEEK and 316L stainless steel HPLC/UHPLC microfluidic manifold blocks. Zero dead volume (<0.03 uL), 0.3mm micro-channels, rated to 400 bar, USP Class VI.
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- Hỗ trợ dung sai ±0.01mm, bao phủ toàn bộ trung tâm gia công CNC 3/4/5 trục
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Product Overview
Engineered for ultra-high-pressure liquid chromatography (HPLC/UHPLC), in-vitro diagnostics (IVD), automated blood analyzers, and precision DNA/RNA synthesis instruments, the Custom PEEK & 316L Stainless Steel HPLC Precision Fluidic Manifold Valve Block (Model: MD-PK-01) delivers inert, zero-dead-volume fluid distribution across hostile solvent pathways.
Machined from virgin medical-grade Polyetheretherketone (PEEK, USP Class VI / ISO 10993) or electro-polished 316L stainless steel, this manifold block features precision CNC micro-drilled intersecting fluid capillaries down to Ø0.3 mm. Designed with conical flat-bottom ports (1/4-28 UNF, 10-32 Coned) and zero-dead-volume intersections (< 0.03 μL), it eliminates sample carryover, reagent cross-contamination, and bubble traps under continuous operating pressures up to 400 bar (5,800 psi).
Figure 1: Custom 7-Port Virgin PEEK HPLC Fluid Distribution Manifold Block with Integrated Finger-Tight Conical Compression Ferrules.
Technical Specifications
| Technical Parameter | Design Specification & Standard |
|---|---|
| Base Manifold Materials | Virgin Unfilled PEEK (Victrex 450G / Ketron 1000, USP Class VI) or Electropolished 316L Stainless Steel / PTFE / PCTFE |
| Micro-Machining Process | Multi-Axis CNC High-Precision Micro-Drilling, Deep-Hole Peck Milling & Mirror Honing |
| Internal Capillary Channel Diameter | Ø0.3 mm ~ Ø2.0 mm (Aspect Ratio up to 30:1 with straightness tolerance ≤ 0.02 mm) |
| Dead Volume / Internal Cavity Holdup | True Zero Dead Volume (< 0.03 μL per channel junction, 100% sweepable flow path) |
| Fluid Pressure Rating | PEEK: Operating up to 350 bar (5,000 psi) | 316L Stainless Steel: up to 700 bar (10,000 psi) Proof tested |
| Internal Channel Surface Roughness | Ra ≤ 0.4 μm (Abrasive flow honed fluid channels to eliminate laminar boundary eddy currents) |
| Chemical Compatibility | 100% Bio-inert across pH 0~14; resistant to acetonitrile, methanol, THF, DMSO, strong acids and bases |
| Port Connection Standards | Standard HPLC 1/4-28 Flat-Bottom, 10-32 Coned, M6, 5/16-24, or Custom Microfluidic Push-Fit Barbs |
Zero-Dead-Volume Microfluidic Architecture
- Computer-Controlled Precision Intersection Geometry: Internal capillaries intersect at mathematically optimized 3D angles to eliminate stagnant dead corners. The sweepable circular cross-section guarantees instantaneous solvent purging during chromatographic gradient changes.
- Multi-Port Radial & Sequential Routing: Configurations support 3-way, 5-way, 7-way, 9-way, or custom multi-layer 24-channel distribution schemes for high-throughput sample preparation and autosampler switching.
- Stress-Relieved Annealing Protocol: All virgin PEEK blanks undergo multi-stage thermal annealing cycles (200 °C slow ramp) before and after CNC machining to relieve polymer internal stresses, preventing dimensional creep or stress cracking over 100,000+ valve actuations.
High-density 9-port radial distribution manifold block for automated multichannel sample splitting.
3-Way PEEK microfluidic Tee fitting with precision knurled compression nuts and conical sealing seats.
Laboratory Metrology & Pressure Validation
- Hydrostatic Proof Testing: 100% of PEEK manifolds are hydrostatically pressure-tested to 1.5× working pressure (500 bar) for 10 minutes with zero external leakage.
- Helium Mass Spectrometer Leak Detection: Tested to ≤ 1×10−8 mbar·L/s ensuring zero cross-channel fluidic bypass between internal micro-channels.
- Fluorescein Dye Carryover Inspection: Validates 99.999% sample flush-out within 3 column volumes to confirm zero dead volume.
- Optical Port Conformance: 100% video optical comparator measurement of 1/4-28 and 10-32 port taper angles and thread pitch diameters.
Frequently Asked Questions (FAQs)
Q1: Why choose PEEK over stainless steel for chromatography fluidic manifolds?
A: PEEK is 100% bio-inert and metal-free, preventing metal ion leaching (iron/nickel) that can poison chromatographic columns or deactivate sensitive biological proteins and oligonucleotides. Furthermore, PEEK resists aggressive halide salts (such as chlorides) that corrode stainless steel.
Q2: What is the smallest internal capillary hole diameter Altus Precision can machine in PEEK?
A: Using specialized micro-carbide drills with optical peck-cycles and high-pressure through-spindle cooling, we routinely machine capillary channels down to Ø0.3 mm (±0.01 mm) with depth-to-diameter aspect ratios up to 30:1, while maintaining Ra ≤ 0.4 μm interior smoothness.
Q3: How do you guarantee true zero dead volume at internal channel intersections?
A: Drill drill-point break-throughs are precisely controlled via 5-axis CAD/CAM coordinate offset calculation, ensuring intersecting channel centerlines meet within ±0.01mm without blind-hole pockets. Abrasive flow extrusion is applied to radiused intersections to eliminate micro-burrs.
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